FastQCFastQC Report
Sat 9 Nov 2024
000000000-LJ9RB_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LJ9RB_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663421
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC744804.4775195215162TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGACATCTCGTATG106260.638804006923082TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGACATCTCGTATG90130.5418351698096874TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGGACATCTCGTATG78510.47197913216197224TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGACATCTCGTATG63190.37987977787944244TruSeq Adapter, Index 2 (97% over 36bp)
TCTCTTCCTCTCCCCCTTCTTCCCTCCCTTCCCCTCCTTCCCTCTCTTCT48410.29102674548415586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC44610.2681822581294813TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTACCGAAGGACATCTCGTATG36730.22081000540452475Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGGACATCTCGTAT22140.1330991973769719TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGGCATCTCGTATG20980.12612561702659758TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCGAAGGACATCTCGTATG19680.11831039766841947TruSeq Adapter, Index 9 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT17070.10261984188007725TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)