FastQCFastQC Report
Sat 9 Nov 2024
000000000-LJ9RB_l01_n01_117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LJ9RB_l01_n01_117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCTTCGGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCATG50.19646365422396855No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGTTAATCTCGTAT40.15717092337917485TruSeq Adapter, Index 9 (97% over 37bp)
GACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGA40.15717092337917485No Hit
ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAA40.15717092337917485No Hit
CATACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTA30.11787819253438114No Hit
TGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTTGG30.11787819253438114No Hit
CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCAC30.11787819253438114No Hit
TAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCAA30.11787819253438114No Hit
GTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCT30.11787819253438114No Hit
GAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACCTGTGA30.11787819253438114No Hit
GTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATAATAACC30.11787819253438114No Hit
AAGCACATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCGCCTCCAA30.11787819253438114No Hit
GACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGA30.11787819253438114No Hit
GAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTA30.11787819253438114No Hit
TCCACGGCGCTTTAAAATAGTTGTTATAGATATTCAAATAACCCTGAAAC30.11787819253438114No Hit
CACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTT30.11787819253438114No Hit
CTTTATCAATACCATGAAAAATATCAACCACACCAGAAGCAGCATCAGTG30.11787819253438114No Hit
TTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACC30.11787819253438114No Hit
TTTAGATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACAT30.11787819253438114No Hit
CGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGC30.11787819253438114No Hit
TAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATA30.11787819253438114No Hit
CGCATAAAGTGCACCGCATGGAAATGAAGACGGCCATTAGCTGTACCATA30.11787819253438114No Hit
ATATCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATT30.11787819253438114No Hit
CACGAAAATAGTCACGCAAAGCATTGGGATTATCATAAAACGCCTCTAAT30.11787819253438114No Hit
AACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCTATTAGTG30.11787819253438114No Hit
TGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCG30.11787819253438114No Hit
CAAATAATCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGCATTT30.11787819253438114No Hit
CGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATA30.11787819253438114No Hit
GGGGGAGCACATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTA30.11787819253438114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)