FastQCFastQC Report
Tue 14 May 2024
000000000-LHRNB_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LHRNB_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561449
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGTTCCGGTGTAGATCTC159432.8396167773030143Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGTCCCGGTGTAGATCTC36030.6417323746235188Illumina Single End PCR Primer 1 (96% over 32bp)
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAAG9130.16261494810748617ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAAG8910.1586965156229684ABI Solid3 Adapter B (100% over 41bp)
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT6130.10918177786406245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)