FastQCFastQC Report
Tue 14 May 2024
000000000-LHRNB_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LHRNB_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561449
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCTCGTATG88771.5810875075029076TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCGATCTCGTATG43690.7781650693117272TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTTGCCATCTCGTATG43390.772821752287385TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAGCTCATCTCGTATG32420.5774344597639323TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC20210.35996145687319775TruSeq Adapter, Index 1 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTCGTATG10490.18683798528450493TruSeq Adapter, Index 10 (97% over 37bp)
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGA9520.16956126023913123No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAGCGAATCTCGTATG9420.16778015456435047TruSeq Adapter, Index 1 (97% over 37bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAAG9400.1674239334293943ABI Solid3 Adapter B (100% over 41bp)
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAAG8710.15513430427340685ABI Solid3 Adapter B (100% over 41bp)
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAACA6000.10686634048684743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)