FastQCFastQC Report
Thu 22 Feb 2024
000000000-LFMD3_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LFMD3_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257202
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCGTAGTGTGTAGATCTC29181.1345168389048297Illumina Single End PCR Primer 1 (96% over 32bp)
AAGGGTTCAGCTTTGTCAACAATGGAGGCAACAACTTTGCTGTGAACATCT26331.023708991376428No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC11200.4354554008133685No Hit
GTACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTAGAGT7440.2892668019688805No Hit
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAAG4960.19284453464592033ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAAG4470.17379336086033548ABI Solid3 Adapter B (100% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCGTAGTGTGTAGAACTC3960.15396458814472672Illumina Single End PCR Primer 1 (96% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.12052783415370019No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCGTAGTGTGTAAATCTC3000.11663983950358084Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)