FastQCFastQC Report
Thu 22 Feb 2024
000000000-LFMD3_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LFMD3_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257202
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG26391.0260417881664996No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCTCGTATG21350.8300868578004836TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTTGCCATCTCGTATG11570.44984098101881015TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCGATCTCGTATG9260.3600283046010529TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAGCTCATCTCGTATG8680.33747793563036055TruSeq Adapter, Index 19 (97% over 37bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC6830.2655500346031524No Hit
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAAG4930.19167813625088453ABI Solid3 Adapter B (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC4850.188567740530789TruSeq Adapter, Index 1 (100% over 51bp)
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAAG4840.1881789410657771ABI Solid3 Adapter B (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTCGTATG2630.10225425929813921TruSeq Adapter, Index 10 (97% over 37bp)
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACT2610.10147666036811533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)