FastQCFastQC Report
Thu 22 Feb 2024
000000000-LFMD3_l01_n01_BS_CR_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LFMD3_l01_n01_BS_CR_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6545326
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAAG380860.581880871938235ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAAG376120.5746390630504883ABI Solid3 Adapter B (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTCGTATG216450.3306939944626135TruSeq Adapter, Index 1 (97% over 36bp)
CTGAGTCGGAGACACGCAGGGATGAGATGGAGATCGGAAGAGCACACGTCT111480.1703200115624493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATCTCATCCCTGCGTGTCTCCGACTCAGAGATCGGAAGAGCACACGTCT106850.16324626153074728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)