FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OODve816.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OODve816.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7304
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC225630.887185104052573No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT129917.784775465498356No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTGGATTAAAACTGTAGACTT107114.663198247535597No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT104014.238773274917854No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT2313.1626506024096384No Hit
AGCCTAGACATCCACTGCTGACGAGTGGTAATGATCCTTCCGCTGTCTCT2002.7382256297918945No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT730.9994523548740416No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT220.30120481927710846No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTGTGGATTAAAACTGTAGACTT210.28751369112814895No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACTT160.21905805038335158No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTGTGGATTAAAACTGTAGACTT160.21905805038335158No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC160.21905805038335158No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC150.20536692223439212No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.17798466593647316No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAGATTAAAACTGTAGACTT120.16429353778751368No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC120.16429353778751368No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.16429353778751368No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC110.15060240963855423No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGTAGAGACT110.15060240963855423No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC90.12322015334063528No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAATAGCAAGGACTT90.12322015334063528No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGTACTT90.12322015334063528No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTGGATTAAAACTGTAGACTC80.10952902519167579No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC80.10952902519167579No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC80.10952902519167579No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC80.10952902519167579No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT80.10952902519167579No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGATAC80.10952902519167579No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC80.10952902519167579No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC80.10952902519167579No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT80.10952902519167579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)