FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OODve767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OODve767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9954
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT530553.295157725537464No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT250525.165762507534662No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT1521.5270243118344384No Hit
AGCCTAGACATCCACTGCTGATGTGGTAATGATCCTTCCGCTGTCTCTTA1471.4767932489451476No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC1231.235684147076552No Hit
AGCCTAGACATCCACTGCTGAAAGTTGATATCTAGTAAAAGCAAGGACTT1161.165360659031545No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAAGACTT1061.0648985332529635No Hit
AGCCTAGACATCCACTGCTGGTGGTAATGATCCTTCCGCTGTCTCTTATA1001.0046212577858147No Hit
AGCCTAGACATACACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT460.46212577858147474No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTGTCTAGTAAAAGCAAGGACTT310.31143258991360256No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT280.2812939521800281No Hit
AGACTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT280.2812939521800281No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAGGCAAGGACTT220.22101667671287922No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT220.22101667671287922No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAGCCAAAGACTT210.21097046413502107No Hit
CGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT210.21097046413502107No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTAAAAGCAAGGACTT190.1908780389793048No Hit
AGCCTAGACATCAACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT180.18083182640144665No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACCT170.1707856138235885No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT160.16073940124573036No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTAAAAGCAAGGACTT140.14064697609001406No Hit
AGCCTATACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT140.14064697609001406No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATTGATTAAAACCAAAGACTT130.1306007635121559No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTC120.12055455093429776No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT110.11050833835643961No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTGGTAAAAGCAAGGACTT110.11050833835643961No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTAAAAGCAAGGACTT100.10046212577858146No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATTGATTAAAACCAAAGACTT100.10046212577858146No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT100.10046212577858146No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTAAAAGCAAGGACTT100.10046212577858146No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATATAGTAAAAGCAAGGACTT100.10046212577858146No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCGAAGACTT100.10046212577858146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)