FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOChi750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOChi750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6438
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT138321.481826654240447No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC119218.51506679092886No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT98015.222118670394533No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC86413.42031686859273No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTATCTAGAATAAAAGTAGAGACT68110.577819198508854No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT1622.516309412861137No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGAATT430.6679092885989437No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTATCTAGAATAAAAGCAGAGACT280.4349176762969867No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT180.27958993476234856No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT130.20192606399502952No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC130.20192606399502952No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.1863932898415657No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC110.1708605156881019No Hit
AGCCTAGACATACACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT100.15532774153463808No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGTAGAGACT90.13979496738117428No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT80.12426219322771047No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT80.12426219322771047No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC80.12426219322771047No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTATCTAGAATAAAAGTAGAGACT80.12426219322771047No Hit
AGACTAGACATCCACTGCTGAAAGTTGTATCTAGAATAAAAGTAGAGACT80.12426219322771047No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAATTAGAGAC80.12426219322771047No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT70.10872941907424667No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGAATT70.10872941907424667No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC70.10872941907424667No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGATAC70.10872941907424667No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATTGATTAAAACCAAAGACTT70.10872941907424667No Hit
AGACTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT70.10872941907424667No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAGAAGCAAGAATT70.10872941907424667No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGATT70.10872941907424667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)