FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOChi728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOChi728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9608
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC590961.500832639467106No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT158216.465445462114907No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT2792.9038301415487093No Hit
AGCCTAGACATCCACTGCTGTTCGTGGTAATGATCCTTCCGCTGTCTCTT780.8118234804329725No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTATCTAGAATAAAAGTAGAGACT660.6869275603663614No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC470.48917568692756036No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC450.4683597002497919No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC440.4579517069109076No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC360.37468776019983346No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC350.3642797668609492No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC300.31223980016652786No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC300.31223980016652786No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC280.29142381348875934No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC270.2810158201498751No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC270.2810158201498751No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT260.2706078268109909No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT260.2706078268109909No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC260.2706078268109909No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC240.2497918401332223No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC230.23938384679433802No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC230.23938384679433802No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTAAACGCAAAAACTTT220.2289758534554538No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC210.21856786011656953No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC200.20815986677768525No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC200.20815986677768525No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC190.19775187343880102No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC180.18734388009991673No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC170.17693588676103247No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC150.15611990008326393No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAGACGCAAAAACTTT140.14571190674437967No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC140.14571190674437967No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC140.14571190674437967No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC130.13530391340549544No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC130.13530391340549544No Hit
AGCCTAGACATCCACTGCTTAAAGTTGTTATCTAGAATAAAAGTAGAGAC130.13530391340549544No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC130.13530391340549544No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC130.13530391340549544No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGTAGAGAC120.12489592006661115No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC120.12489592006661115No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAT110.1144879267277269No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGTAGAATAAAAGTAGAGAC110.1144879267277269No Hit
AGCCTAGACATCCACTGTTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.1144879267277269No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGATAC110.1144879267277269No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTATAGAC100.10407993338884262No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGAATAAAAGTAGAGAC100.10407993338884262No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTAAACGCAAAAACTTT100.10407993338884262No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC100.10407993338884262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)