FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOChi727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOChi727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12650
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT477137.715415019762844No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC183014.466403162055336No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT174213.770750988142291No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT9297.3438735177865615No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT5384.252964426877471No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT2081.6442687747035574No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT1250.9881422924901186No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT780.616600790513834No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT380.30039525691699603No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTGAACGCAAAAACTTT360.2845849802371541No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT360.2845849802371541No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT310.2450592885375494No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAGAGCAAGGACTT300.23715415019762848No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT280.22134387351778656No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT270.21343873517786563No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT260.20553359683794467No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT260.20553359683794467No Hit
AGCCTAGACATCCACTGCTGGAAGTTTGTGGAAGTGAACGCAAAAACTTT250.1976284584980237No Hit
AGCCTAGACATCCACTGCTGAGAGTTTGTGGAAGTGAACGCAAAAACTTT250.1976284584980237No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT240.18972332015810275No Hit
AGCCTAGACATCCACTGCTGAAATTTGTTATCCAGAATAAAATTAGAGAC240.18972332015810275No Hit
AGCCTAGACATCCACTGCTGTCTCTTATACACATCTGACGCTGCCGACGA230.18181818181818182No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTCT230.18181818181818182No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT220.17391304347826086No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCGAAAACTTT220.17391304347826086No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAGAACTTT220.17391304347826086No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACATT210.1660079051383399No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTTAACGCAAAAACTTT200.15810276679841898No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT200.15810276679841898No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACACAAAAACTTT190.15019762845849802No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT180.14229249011857706No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC180.14229249011857706No Hit
CGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT150.11857707509881424No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAGCTTT150.11857707509881424No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATATAAGCAGAGAC140.11067193675889328No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC140.11067193675889328No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT140.11067193675889328No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT130.10276679841897234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)