FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOATH659.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOATH659.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14756
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC1252784.89428029276228No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC1080.7319056654920032No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC620.42016806722689076No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC570.38628354567633505No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC450.30496069395500136No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC450.30496069395500136No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAATTAGAGAC420.2846299810246679No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC410.2778530767145568No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC400.27107617240444565No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC400.27107617240444565No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC390.2642992680943345No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC390.2642992680943345No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC380.2575223637842234No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC360.2439685551640011No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC330.22363784223366767No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGATAC330.22363784223366767No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC320.21686093792355648No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC310.21008403361344538No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC310.21008403361344538No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC290.19653022499322312No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC290.19653022499322312No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC290.19653022499322312No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC280.18975332068311196No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC280.18975332068311196No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC280.18975332068311196No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC270.1829764163730008No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGCAGAGAC260.17619951206288967No Hit
AGCCTAGACATCCACTGCTTAAAGTTGTTATCTAGAATAAAAGCAGAGAC250.16942260775277854No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC240.1626457034426674No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC240.1626457034426674No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCATAGAC240.1626457034426674No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC210.14231499051233396No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT210.14231499051233396No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC210.14231499051233396No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC200.13553808620222282No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGCAGAGAC190.1287611818921117No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC190.1287611818921117No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC190.1287611818921117No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC180.12198427758200055No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTTAGAATAAAAGCAGAGAC170.1152073732718894No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC170.1152073732718894No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAAAGAC170.1152073732718894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)