FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOATH643.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOATH643.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13166
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT1145186.97402400121526No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT1050.7975087346194744No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT540.4101473492328725No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTTAACGCAAAAACTTT500.37976606410451164No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT490.37217074282242135No Hit
CGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT460.3493847789761507No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT420.31900349384778975No Hit
AGCCTAGAAATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT420.31900349384778975No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT340.2582409235910679No Hit
AGCCTATACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT310.23545495974479722No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT290.22026431718061676No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT260.19747835333434605No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT230.17469238948807536No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT220.16709706820598513No Hit
AGCATAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT220.16709706820598513No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGTAAGTGAACGCAAAAACTTT200.15190642564180465No Hit
AGCCTAGATATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT190.14431110435971442No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT190.14431110435971442No Hit
AGCCTAGACATCCACTGCTTAAAGTTTGTGGAAGTGAACGCAAAAACTTT180.13671578307762416No Hit
AGCCTAGACATTCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT170.12912046179553394No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT170.12912046179553394No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCGAAAACTTT170.12912046179553394No Hit
AGCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC160.12152514051344372No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC160.12152514051344372No Hit
AGCCTAGACATCTACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT150.11392981923135348No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT150.11392981923135348No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGCAGTGAACGCAAAAACTTT150.11392981923135348No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTTGAAGTGAACGCAAAAACTTT150.11392981923135348No Hit
AGTCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT140.10633449794926325No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTCT140.10633449794926325No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT140.10633449794926325No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)