FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOATH642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOATH642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5855
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT190232.48505550811272No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT145624.867634500426984No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC122720.956447480785652No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAATCAGAGAC691.1784799316823227No Hit
AGCCTAGACATCCACTGCTGGTGGTAATGATCCTTCCGCTGTCTCTTATA490.8368915456874466No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC460.7856532877882152No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAATTATAGAC460.7856532877882152No Hit
AGCCTAGACATCCACTGCTGATGTGGTAATGATCCTTCCGCTGTCTCTTA450.7685738684884714No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT330.5636208368915456No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC260.4440649017933391No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT170.29035012809564475No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT150.25619128949615716No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT130.22203245089666954No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT120.2049530315969257No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT110.18787361229718189No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC110.18787361229718189No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC110.18787361229718189No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGGCTT110.18787361229718189No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT110.18787361229718189No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT90.1537147736976943No Hit
AGCCTAGACATCCACTGCTGAAAGTGTTTTGACATTGTTCTTTAGCCAGA90.1537147736976943No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT90.1537147736976943No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAGACGCAAAAACTTT90.1537147736976943No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC90.1537147736976943No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC90.1537147736976943No Hit
AGCCTAGACATCCACTGCTGGAAGTTTGTGGAAGTGAACGCAAAAACTTT70.11955593509820667No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.11955593509820667No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT70.11955593509820667No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.11955593509820667No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTAAACGCAAAAACTTT70.11955593509820667No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC70.11955593509820667No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTAAACGCAAAAACTTT70.11955593509820667No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC60.10247651579846286No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC60.10247651579846286No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTAAATGAATTTCTAGTTTGCGTG60.10247651579846286No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGCAGAGAC60.10247651579846286No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAGACTTT60.10247651579846286No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT60.10247651579846286No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC60.10247651579846286No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTTGAAGTGAACGCAAAAACTTT60.10247651579846286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)