FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOATH631.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOATH631.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8517
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC647776.04790419161677No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT4955.811905600563579No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC500.5870611717740989No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC330.3874603733709053No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC320.37571914993542327No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC320.37571914993542327No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC290.3404954796289773No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC280.32875425619349535No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC270.3170130327580134No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC260.3052718093225314No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC260.3052718093225314No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC250.29353058588704944No Hit
AGCCTAGACATCCACTGCTGTGTGGTAATGATCCTTCCGCTGTCTCTTAT250.29353058588704944No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC250.29353058588704944No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGATAC240.28178936245156744No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC240.28178936245156744No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT220.2583069155806035No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC210.24656569214512153No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGCAGAGAC200.23482446870963955No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC190.2230832452741576No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC190.2230832452741576No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC180.21134202183867556No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC180.21134202183867556No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC180.21134202183867556No Hit
AGCCTAGACATCCACTGCTGGAGATGTGGTAATGATCCTTCCGCTGTCTC180.21134202183867556No Hit
AGCCTAGACATCCACTGCTTAAAGTTGTTATCTAGAATAAAAGCAGAGAC170.19960079840319359No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC160.18785957496771163No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC160.18785957496771163No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC150.17611835153222966No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC150.17611835153222966No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC140.16437712809674768No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC140.16437712809674768No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCATAGAC140.16437712809674768No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.1526359046612657No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.1526359046612657No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.1526359046612657No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.14089468122578372No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC120.14089468122578372No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.14089468122578372No Hit
AGCCTAGACATCCACTGCTGAAATTTGTTATCCAGAATAAAATTAGAGAC100.11741223435481977No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT100.11741223435481977No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGAAGAGAC100.11741223435481977No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGCAGAGAC90.10567101091933778No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAAAC90.10567101091933778No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT90.10567101091933778No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAAAGAC90.10567101091933778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)