FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOATH605.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOATH605.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10692
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC940988.00037411148523No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC710.6640478862701085No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC460.4302282080059858No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC410.3834642723531612No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGATAC310.2899364010475121No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC300.28058361391694725No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC270.25252525252525254No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC240.22446689113355783No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC230.2151141040029929No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC230.2151141040029929No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC230.2151141040029929No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC220.205761316872428No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC210.1964085297418631No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGCAGAGAC190.17770295548073325No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC190.17770295548073325No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC180.16835016835016833No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC180.16835016835016833No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT180.16835016835016833No Hit
AGCCTAGACATCCACTGCTTAAAGTTGTTATCTAGAATAAAAGCAGAGAC180.16835016835016833No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC170.15899738121960344No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC160.14964459408903852No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC160.14964459408903852No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCATAGAC160.14964459408903852No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC160.14964459408903852No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT140.1309390198279087No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT140.1309390198279087No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT140.1309390198279087No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.12158623269734382No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC130.12158623269734382No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAAAGAC120.11223344556677892No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGAAGAGAC110.102880658436214No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC110.102880658436214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)