FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOATH589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOATH589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4767
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC228547.93371092930565No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT142829.955947136563875No Hit
AGCCTAGACATCCACTGCTGCCGAGTGGTAATGATCCTTCCGCTGTCTCT1002.097755401720159No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC651.3635410111181034No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC160.3356408642752255No Hit
AGCCTAGACATCCACTGCTGCAGCGTCAGATGTGGTAATGATCCTTCCGC150.3146633102580239No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC140.2936857562408223No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC130.27270820222362074No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.27270820222362074No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT120.25173064820641916No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT110.23075309418921752No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGTACT110.23075309418921752No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAAGGACT110.23075309418921752No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC110.23075309418921752No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC100.20977554017201594No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC100.20977554017201594No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC100.20977554017201594No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC90.18879798615481436No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACC90.18879798615481436No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTTAAAAGCAAGGACT90.18879798615481436No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC90.18879798615481436No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAATGCAAGGACT80.16782043213761275No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC80.16782043213761275No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC80.16782043213761275No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.16782043213761275No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT80.16782043213761275No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC80.16782043213761275No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.16782043213761275No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT80.16782043213761275No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT70.14684287812041116No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGCAGAGAC70.14684287812041116No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC70.14684287812041116No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAAGGACT70.14684287812041116No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT70.14684287812041116No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGCAGAGAC70.14684287812041116No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGGACT70.14684287812041116No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGGACT70.14684287812041116No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAGAGCAAGGACT60.12586532410320958No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC60.12586532410320958No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.12586532410320958No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC60.12586532410320958No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC60.12586532410320958No Hit
AGCCTAGACATCCACTGCTTAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.12586532410320958No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGGCT50.10488777008600797No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC50.10488777008600797No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT50.10488777008600797No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC50.10488777008600797No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC50.10488777008600797No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC50.10488777008600797No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT50.10488777008600797No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC50.10488777008600797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)