FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n02_ITS-OOATH574.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n02_ITS-OOATH574.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12071
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC868971.98243724629276No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT141311.705741032225996No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT2301.9053930908789662No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC620.5136277027586779No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC500.41421588932151443No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC340.28166680473862976No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC330.27338248695219947No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC330.27338248695219947No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC290.24024521580647834No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC290.24024521580647834No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC290.24024521580647834No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC260.21539226244718748No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC250.20710794466075721No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC250.20710794466075721No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC250.20710794466075721No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT230.1905393090878966No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC230.1905393090878966No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC230.1905393090878966No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC230.1905393090878966No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC220.1822549913014663No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCATAGAC200.16568635572860577No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGCAGAGAC190.15740203794217547No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC180.14911772015574518No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC170.14083340236931488No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC160.13254908458288459No Hit
AGCCTAGACATCCACTGCTTAAAGTTGTTATCTAGAATAAAAGCAGAGAC160.13254908458288459No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC160.13254908458288459No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGCAGAGAC150.12426476679645432No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC150.12426476679645432No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC150.12426476679645432No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC150.12426476679645432No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAAAGAC150.12426476679645432No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC140.11598044901002402No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC140.11598044901002402No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC140.11598044901002402No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT140.11598044901002402No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAATTAGAGAC140.11598044901002402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)