FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n01_ITS-OOATH632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n01_ITS-OOATH632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10005
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT875187.46626686656673No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT550.5497251374312844No Hit
CGGAATGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT500.49975012493753124No Hit
CGGAAGTATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT400.39980009995002497No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT350.3498250874562719No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT310.3098450774612694No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACAACTTTGTAT290.2898550724637681No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT280.2798600699650175No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT280.2798600699650175No Hit
CGTAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT250.24987506246876562No Hit
CGGAAGGATCATTACCAAACCAAAAACTATCCACGTGAACCACTTTGTAT250.24987506246876562No Hit
CGGAAGGATCATTACAACACCAAAAACTATCCACGTGAACCACTTTGTAT250.24987506246876562No Hit
CTGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT240.23988005997001496No Hit
CGGAAGGATAATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT210.2098950524737631No Hit
CGGAAGGATCATTACCACACAAAAAACTATCCACGTGAACCACTTTGTAT200.19990004997501248No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCGCGTGAACCACTTTGTAT200.19990004997501248No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT200.19990004997501248No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCAATTTGTAT190.18990504747626188No Hit
CGGAAGGATCATTACCACACCGAAAACTATCCACGTGAACCACTTTGTAT190.18990504747626188No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT180.17991004497751123No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACCTTGTAT180.17991004497751123No Hit
CGGAAGGATCATTACCACACCAAAAACTACCCACGTGAACCACTTTGTAT160.15992003998001No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT160.15992003998001No Hit
CGGAAGGATCATTACCACACCAAGAACTATCCACGTGAACCACTTTGTAT150.14992503748125938No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAATCACTTTGTAT140.13993003498250875No Hit
TGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT130.12993503248375812No Hit
CGGAAGGATCATTACCACACCAAAAACTGTCCACGTGAACCACTTTGTAT130.12993503248375812No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCATGTGAACCACTTTGTAT120.11994002998500748No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTAAACCACTTTGTAT120.11994002998500748No Hit
CGGAAGGATCATTACCACACCAAAAACTATTCACGTGAACCACTTTGTAT110.10994502748625688No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT110.10994502748625688No Hit
CGGAAGGATCATTACCACACCAAAAGCTATCCACGTGAACCACTTTGTAT110.10994502748625688No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)