FastQCFastQC Report
Mon 29 Jan 2024
000000000-LDHC6_l01_n01_ITS-OOATH625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LDHC6_l01_n01_ITS-OOATH625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5694
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTTGTAC193133.91289076220583No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAAGC71712.592202318229715No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGTTAACCAA5509.65929048120829No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT5239.18510713031261No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGTTAAGCAA4658.166491043203372No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGACCCGTTAAGC811.422550052687039No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGTTAATCAA791.3874253600281No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTTGTA571.0010537407797682No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC500.8781173164734809No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA250.43905865823674045No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGTTTGTACC240.42149631190727077No Hit
CGGAAGGATCATTACCACACCTAAAAAACTCTCCACGTGAACCGTTAAGC220.3863716192483316No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACCGTTACATA220.3863716192483316No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTTCACGTGAACCGTTTAATA220.3863716192483316No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT200.3512469265893923No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCACTTTGT190.3336845802599227No Hit
CGGAAGGATCATTACCACACCAAAAACAATCCACGTGAACCGTCAGAAAT180.31612223393045313No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGGACCGTTTGTAC180.31612223393045313No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCATGTGAACCGTTCTAC180.31612223393045313No Hit
CGGAAGGATCATTACCACACCTAAAAACCTATCCACGTGAACCGTTAAGC170.2985598876009835No Hit
CGGAAGGATCATTACCACATCCAGCAGTGGATGTCTAGGCTCTGTCTCTT170.2985598876009835No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAACC160.2809975412715139No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGCGAACCGTTTGTAC140.24587284861257463No Hit
CGGAAGGATCATTACCACACCTAAAAAACTCTCCACGTGACCCGTTAAGC130.228310502283105No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTAAGCA130.228310502283105No Hit
CGGAAGGATCATTACCACACCTTAAAGCTATCCACGTGAACCGTTTGTAC120.21074815595363539No Hit
CGGAAGGATCATTACCACACCTTAAAACTACCCACGTGAACCGTTTGTAC110.1931858096241658No Hit
CGGAAGGATCATTACCACGCCTTAAAACTATCCACGTGAACCGTTTGTAC110.1931858096241658No Hit
CGGAAGGATCATTACCACTGTCTCTTACAGCAGTGGATGTCTAGGCTCTG110.1931858096241658No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGACCCGTTTGTA100.17562346329469616No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTTGTGC90.15806111696522657No Hit
CGGAAGTATCATTACCACACCTTAAAACTATCCACGTGAACCGTTTGTAC90.15806111696522657No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTTAACCAA90.15806111696522657No Hit
CGGAAGGATCATTACCACACCTTAAAAATATCCACGTGAACCGTTTGTAC80.14049877063575694No Hit
CGGAAGGATTATTACCACACCTTAAAACTATCCACGTGAACCGTTTGTAC70.12293642430628732No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACAGTTTGTAC70.12293642430628732No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTTAACCGTTTGTAC70.12293642430628732No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGCTTGTAC70.12293642430628732No Hit
CTGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTTGTAC70.12293642430628732No Hit
CGGAAGGATAATTACCACACCTTAAAACTATCCACGTGAACCGTTTGTAC60.10537407797681769No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCGTTAAGCA60.10537407797681769No Hit
CGGAATGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTTGTAC60.10537407797681769No Hit
CGGAAGGATCATTACCACACCTTAAGACTATCCACGTGAACCGTTTGTAC60.10537407797681769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)