FastQCFastQC Report
Wed 5 Jun 2024
000000000-LD82W_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LD82W_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8580157
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC273816231.912726072494944Illumina Single End PCR Primer 1 (100% over 8bp)
TCTACACT3448414.019052332026092Illumina Single End PCR Primer 1 (100% over 8bp)
GTCTAGTG3408303.9723049356789155No Hit
CTAGTATG3279453.8221328584080685No Hit
GATAGCGT3002843.4997494801085804No Hit
ACGCGTGA2794663.2571198872001994No Hit
CGATCTAC2784553.245336886026678No Hit
AAGCAGCA2738533.191701503830291No Hit
TGCGTCAC2305162.686617505950066No Hit
CTACTATA1652731.9262234945118135No Hit
GTCAGATA1625971.8950352540169135No Hit
CGTTACTA1531511.784944028413466No Hit
CTGCGTGT1253541.4609755975327723No Hit
ATCGTACG1186631.3829933414971312RNA PCR Primer, Index 22 (100% over 8bp)
ACCTGGTT1139881.3285071590181858No Hit
TCATCGAG1139861.3284838494214033No Hit
TAGCGAGT1106711.289848192754515No Hit
GATCGTGT1092651.273461546216462No Hit
CGTCGCTA1005671.1720881098096456No Hit
TGCGTACG996931.1619018160157208No Hit
GACACCGT949021.1060636769233942No Hit
CGTGAGTG948221.105131293052097No Hit
GACACTGA924611.0776143140504306No Hit
CTAGAGCT923251.0760292614692248No Hit
TCGACGAG912531.063535317593839No Hit
TAGTGTAG882681.0287457443960524No Hit
GGATATCT869901.0138509120520756No Hit
ACTATCTG868771.0125339198338679No Hit
ACGACGTG847440.9876742348653994No Hit
AGAGTCAC835100.973292213650636No Hit
ACGTCTCG747850.8716040976872569No Hit
GCTCTAGT735690.8574318628435353No Hit
ATATACAC728680.8492618491712913No Hit
TACGAGAC589490.6870387103639246TruSeq Adapter, Index 14 (100% over 8bp)
TCTTTCAC116380.13563854367699799No Hit
TCGGTTCA99640.11612841117009864No Hit
AGGTTGCA97200.11328464036264138No Hit
TGCGTGAA97080.11314478278194676No Hit
TCTCTGGA90370.10532441306143933No Hit
TCCAACAG88380.1030051081815869No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)