FastQCFastQC Report
Wed 5 Jun 2024
000000000-LD82W_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LD82W_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8580157
Sequences flagged as poor quality0
Sequence length8
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACTG2442542.8467311262486223No Hit
GACATAGT2402762.800368338248356No Hit
CGAGAGTT2253222.6260824831060785TruSeq Adapter, Index 13 (100% over 8bp)
TGAGTACG2011332.3441645648209No Hit
CTGCGTAG1902942.2178382050584857No Hit
GTCTATGA1900892.2154489713882857No Hit
ACGCTACT1871172.180810910569585No Hit
TAGTCTCC1869582.1789577976253813No Hit
GTCTGCTA1809962.1094718896169384No Hit
ACTACGAC1721832.0067581513951316No Hit
TAGCAGCT1460831.7025679133843354No Hit
CGAGCGAC1407061.639900062434755No Hit
ATAGTACC1388691.618490197790087No Hit
GATCTACG1310201.5270116852174151No Hit
CTCGACTT1297051.511685625332963No Hit
CGAAGTAT1244241.4501366350289395No Hit
TATAGCGA1242741.4483884152702566No Hit
CGTCATAC1175291.3697768001214896No Hit
AACGCTGA1151771.3423647143053443No Hit
ACGTGCGC1131271.318472377603347No Hit
TCTCTATG921371.0738381593716757ABI Solid3 Adapter B (100% over 8bp)
ATGAGCTC791900.9229434846005733No Hit
GTAACGAG780640.9098201816120614No Hit
ATAGCGCT762760.8889814020885631No Hit
AGCATACC761350.8873380755154014No Hit
TGCTCGTA738050.860182395263863No Hit
CTCTAGAG718590.8375021575945522No Hit
CAGTAGGT702220.8184232526281279No Hit
GCGTATAC689610.8037265518568016No Hit
TCTAGACT683820.7969784235882862No Hit
ACGTACGT668210.7787852832995946No Hit
GAGCTCGA620690.7234016813445255No Hit
AGTCGCAG606650.7070383444032551No Hit
ACTGTGTA595770.6943579237536097No Hit
CTACGACC565470.6590438846282184No Hit
TCAGTCTA558140.6505009174074553No Hit
GTTACAGC548910.639743538492361No Hit
TACTAGGT536020.6247205033660806No Hit
CGCGATAT534200.6225993300588789No Hit
CGAGCTAG531350.6192777125173816No Hit
CTATCGTG530470.6182520902589544No Hit
TAACGTCC522840.6093594790864549No Hit
CGTAGCGA488950.5698613673386163No Hit
GAGACTTA461810.53823024450485No Hit
TCCTCATG451620.5263540049441986No Hit
GCGATACG446140.519967175425811No Hit
CATTACGG362130.42205521414118646No Hit
TGCGTCAA354970.4137103784930741No Hit
GATTCGGA328090.38238228041747957No Hit
GCGACTAA324740.3784779229564214No Hit
ACAGTGGT304570.3549701946013342No Hit
AAGCGACT301530.35142713589040386No Hit
AAGCGTTC265740.30971461244823373No Hit
GGTTGCAA253680.29565892558842455No Hit
GGATCTGA252230.2939689798216979No Hit
AAGGCGTA217740.25377158017038615No Hit
GAACCACA210440.24526357734479684No Hit
TTTTTTTT203440.23710521847094407No Hit
TGATCACG197160.229786005081259No Hit
CATCGTGA185580.21628974854422828No Hit
AAAAAAAA179650.20937845309823583No Hit
CATCTTCG176930.20620834793582446No Hit
CGTGTACA126290.14718844888269528No Hit
AACATAGT86050.10028954015643303No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)