FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OODve850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OODve850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC253282.85340314136126No Hit
AGCCTAGACATCCACTGCTGTGTGGTAATGATCCTTCCGCTGTCTCTTAT280.9162303664921465No Hit
AGCCTAGACATCCACTGCTGGGAGATGTGGTAATGATCCTTCCGCTGTCT240.7853403141361256No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC160.5235602094240838No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC160.5235602094240838No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC130.42539267015706805No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC110.3599476439790576No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC100.32722513089005234No Hit
AGCCTAGACATCCACTGCTGTTCGTGGTAATGATCCTTCCGCTGTCTCTT100.32722513089005234No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC100.32722513089005234No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC90.29450261780104714No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC80.2617801047120419No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.2617801047120419No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC80.2617801047120419No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC80.2617801047120419No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC80.2617801047120419No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC70.22905759162303663No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC70.22905759162303663No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC70.22905759162303663No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.1963350785340314No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.1963350785340314No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.1963350785340314No Hit
AGCCTAGACATCCCTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT50.16361256544502617No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC50.16361256544502617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC50.16361256544502617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC50.16361256544502617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAA40.13089005235602094No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC40.13089005235602094No Hit
AGCCTAGACATCCACTGCTGAAAGTTGGTATCTAGAATAAAAGCAGAGAC40.13089005235602094No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.13089005235602094No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC40.13089005235602094No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTTAGAATAAAAGCAGAGAC40.13089005235602094No Hit
AGCCTAGACATCCACTGCTGGGAGGTGTGGTAATGATCCTTCCGCTGTCT40.13089005235602094No Hit
AGCCTAAACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.13089005235602094No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.13089005235602094No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGACTAAAAGCAGAGAC40.13089005235602094No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)