FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OODve835.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OODve835.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6462
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT350154.1782729805014No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT150323.25905292479109No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT1772.7390900649953576No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT1372.1200866604766326No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC500.773754255648406No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTCT490.7582791705354379No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT300.4642525533890436No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAGAGACT290.4487774682760755No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT210.32497678737233054No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT210.32497678737233054No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT200.3095017022593624No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT200.3095017022593624No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT200.3095017022593624No Hit
AGCCTAGACATCCACTGCTGGAAGTTTGTGGAAGTGAACGCAAAAACTTT190.2940266171463943No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT170.26307644692045806No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGGCT150.2321262766945218No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAGAACTTT150.2321262766945218No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTGAACGCAAAAACTTT150.2321262766945218No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT140.2166511915815537No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAGCTTT140.2166511915815537No Hit
AGCCTAGACATCCACTGCTGAGAGTTTGTGGAAGTGAACGCAAAAACTTT140.2166511915815537No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT130.20117610646858555No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT130.20117610646858555No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT120.18570102135561745No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT120.18570102135561745No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT120.18570102135561745No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGGAGACT110.17022593624264934No Hit
AGCCTAGACATCCACTGCTGAAAGCTTGTGGAAGTGAACGCAAAAACTTT100.1547508511296812No Hit
AGCCTAGAAATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT100.1547508511296812No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAACAACTTT90.1392757660167131No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC90.1392757660167131No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAGAGACT80.12380068090374496No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACC80.12380068090374496No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAGAGACT70.10832559579077684No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT70.10832559579077684No Hit
AGCCTAGACACCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC70.10832559579077684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)