FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OODve815.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OODve815.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4856
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC429488.42668863261945No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGTC220.4530477759472817No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC190.39126853377265236No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC180.37067545304777594No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC180.37067545304777594No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC160.3294892915980231No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC140.2883031301482702No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC140.2883031301482702No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC120.2471169686985173No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.22652388797364084No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGCAGAGAC110.22652388797364084No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGCC100.20593080724876442No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGCAGAGAC100.20593080724876442No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT90.18533772652388797No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.16474464579901155No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC80.16474464579901155No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.16474464579901155No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC80.16474464579901155No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC80.16474464579901155No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC70.1441515650741351No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC70.1441515650741351No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC70.1441515650741351No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC70.1441515650741351No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC70.1441515650741351No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC70.1441515650741351No Hit
AGCCTAGACATCCACTGCCGAAAGTTGTTATCTAGAATAAAAGCAGAGAC70.1441515650741351No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT60.12355848434925865No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC50.10296540362438221No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC50.10296540362438221No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT50.10296540362438221No Hit
GAGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGA50.10296540362438221No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC50.10296540362438221No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)