FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OODve814.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OODve814.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7274
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC315943.4286499862524No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT315743.401154797910365No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT180.2474566950783613No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC170.2337091009073412No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC160.21996150673632117No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC140.192466318394281No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT130.17871872422326093No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT130.17871872422326093No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC120.16497113005224084No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.16497113005224084No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT120.16497113005224084No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAGAACTTT120.16497113005224084No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC110.15122353588122078No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT110.15122353588122078No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT110.15122353588122078No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC100.13747594171020072No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT100.13747594171020072No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC100.13747594171020072No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC100.13747594171020072No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT100.13747594171020072No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTGAACGCAAAAACTTT100.13747594171020072No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC90.12372834753918065No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT90.12372834753918065No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT90.12372834753918065No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.10998075336816059No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC80.10998075336816059No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT80.10998075336816059No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC80.10998075336816059No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC80.10998075336816059No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC80.10998075336816059No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCGAAAACTTT80.10998075336816059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)