FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OODve813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OODve813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4430
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC389187.83295711060947No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC160.3611738148984198No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC150.33860045146726864No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC140.3160270880361174No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC130.2934537246049661No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC120.27088036117381487No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC120.27088036117381487No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC100.2257336343115124No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC100.2257336343115124No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC100.2257336343115124No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC100.2257336343115124No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC100.2257336343115124No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC100.2257336343115124No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGCAGAGAC90.20316027088036118No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATCAAAGCAGAGAC90.20316027088036118No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC90.20316027088036118No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC90.20316027088036118No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATACAAGCAGAGAC80.1805869074492099No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC80.1805869074492099No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.1805869074492099No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC80.1805869074492099No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.1805869074492099No Hit
AGCCTAGACATCCATTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC70.1580135440180587No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC70.1580135440180587No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC60.13544018058690743No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.13544018058690743No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGCAGAGAC60.13544018058690743No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.13544018058690743No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC60.13544018058690743No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGAATAAAAGCAGAGAC50.1128668171557562No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC50.1128668171557562No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAAAC50.1128668171557562No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGAAGAGAC50.1128668171557562No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCATAAAAGCAGAGAC50.1128668171557562No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC50.1128668171557562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)