FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OODve806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OODve806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT215250.457209847596715No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC101923.892145369284876No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT3959.261430246189919No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT561.3130128956623681No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC551.2895662368112544No Hit
AGCCTAGACATCCACTGCTGAAGTGGTAATGATCCTTCCGCTGTCTCTTA240.5627198124267292No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC180.4220398593200469No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTCAATTAAAAGCAAGAATT110.2579132473622509No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATTTAATTAAAAGCAAGAATT110.2579132473622509No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAGGAATT100.23446658851113714No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCGAGAATT100.23446658851113714No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATTTAATTAAAAGCAAGAATT100.23446658851113714No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC90.21101992966002345No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATTTAATTAAAAGCAAGAATT90.21101992966002345No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAGAAGCAAGAATT80.18757327080890973No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGGATT80.18757327080890973No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAGAGCAAGAATT80.18757327080890973No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAGTT80.18757327080890973No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATTTAATTAAAAGCAAGAATT70.16412661195779601No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATC70.16412661195779601No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT70.16412661195779601No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC70.16412661195779601No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATTTAATTAAAAGCAAGAATT70.16412661195779601No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATTTAATTAAAAGCAAGAATT70.16412661195779601No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTTAAAAGCAAGAATT60.1406799531066823No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC60.1406799531066823No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC60.1406799531066823No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC60.1406799531066823No Hit
AGACTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT60.1406799531066823No Hit
AGCCTAGATATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT60.1406799531066823No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTACAAGCAAGAATT50.11723329425556857No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC50.11723329425556857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)