FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OODve780.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OODve780.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length301
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC2863.63636363636363No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA36.8181818181818175No Hit
TGAGTACCCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA12.272727272727273No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCACAAACTTT12.272727272727273No Hit
GCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT12.272727272727273No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGACTAAAAGCAGAGAC12.272727272727273No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGGATTAAAACCAAAAACTT12.272727272727273No Hit
AGCCTAGACATCCACTGCTGAAAGTTTTTGTCAGGTAACACAAAAACCTT12.272727272727273No Hit
AGCCTAGACTCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT12.272727272727273No Hit
TGAGTAACCGCAACATCGTGAAAACACAGTTATCCATACTAGTTAGAGAA12.272727272727273No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAACGCAGAGAC12.272727272727273No Hit
AGCTTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC12.272727272727273No Hit
ACGCCGTGGAGGCCGCCAGACACCTTGTAGCCGGCGCCGCCGAACTTGCC12.272727272727273No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA12.272727272727273No Hit
TGAGTAACCGCACCATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)