FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8626
Sequences flagged as poor quality0
Sequence length301
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC740285.81034083004869No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT580.67238581034083No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC360.41734291676327384No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC350.405750057964294No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC340.3941571991653142No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC290.336192905170415No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC260.3014143287734755No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC250.2898214699744957No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC250.2898214699744957No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC230.26663575237653603No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC230.26663575237653603No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC230.26663575237653603No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC200.2318571759795966No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC200.2318571759795966No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC190.22026431718061676No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC180.20867145838163692No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC180.20867145838163692No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC170.1970785995826571No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC170.1970785995826571No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC150.17389288198469743No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGCC150.17389288198469743No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGTAGAGAC150.17389288198469743No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC140.1623000231857176No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC140.1623000231857176No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC130.15070716438673776No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC130.15070716438673776No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC120.13911430558775792No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT120.13911430558775792No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC120.13911430558775792No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC120.13911430558775792No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT120.13911430558775792No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.1275214467887781No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATCCTTCCGCTGTCTCTT100.1159285879897983No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGACTAAAAGTAGAGAC90.10433572919081846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATACAAGTAGAGAC90.10433572919081846No Hit
AGCCTAGACTCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT90.10433572919081846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC90.10433572919081846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATCAAAGTAGAGAC90.10433572919081846No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT90.10433572919081846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)