FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi747.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi747.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT198340.13357619914997No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT87017.60777170613236No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT50110.139647844565877No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC4158.399109492005667No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC1713.4608378870673953No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT961.9429265330904677No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC611.2345679012345678No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATCCTTCCGCTGTCTCTT210.42501517911353975No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT200.40477636106051407No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT140.28334345274235984No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGGCTCGGAGATGTGGTAATGATC140.28334345274235984No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTTAAAAGCAAGGACT110.22262699858328272No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT110.22262699858328272No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGGATTAAAACCAAAAACTT100.20238818053025703No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT100.20238818053025703No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT100.20238818053025703No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAAGGACT90.18214936247723132No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAAGGACT90.18214936247723132No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT80.16191054442420563No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT80.16191054442420563No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGGACT80.16191054442420563No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAGGCAGGGACTT70.14167172637117992No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.14167172637117992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT70.14167172637117992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGAGACTT70.14167172637117992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGGACT70.14167172637117992No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGTTAAAAGCAAGGACT60.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT60.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT60.12143290831815423No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTAAAAGCAGGGACTT60.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAGAAGCAAGGACT60.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT50.10119409026512852No Hit
AGCCTAGACATCCACTGCTGAAAGTTATTATCTAGTTAAAAGCAAGGACT50.10119409026512852No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC50.10119409026512852No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC50.10119409026512852No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTTAAAAGCAAGGACT50.10119409026512852No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)