FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi730.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi730.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC300777.72034117342983No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC1493.851124321530111No Hit
AGCCTAGACATCCACTGCTGAAAGTTATTATTGATTAAACAGACGACTTT280.7237012147841819No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT220.5686223830447144No Hit
AGCCTAGACATCCACTGCTGGTTCGTGGTAATGATCCTTCCGCTGTCTCT210.5427759110881365No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT200.5169294391315585No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC190.4910829671749806No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC180.4652364952184027No Hit
AGCCTAGACATCCACTGCTGGATGTGGTAATGATCCTTCCGCTGTCTCTT170.43939002326182475No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC160.41354355130524684No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC160.41354355130524684No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC150.3876970793486689No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC140.36185060739209096No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC130.33600413543551305No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC120.31015766347893514No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC120.31015766347893514No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC120.31015766347893514No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC120.31015766347893514No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC110.2843111915223572No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC110.2843111915223572No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC100.25846471956577927No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC100.25846471956577927No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC100.25846471956577927No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC100.25846471956577927No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC90.23261824760920136No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC90.23261824760920136No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGTAGAGAC80.20677177565262342No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC80.20677177565262342No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC80.20677177565262342No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGTAGAGAC80.20677177565262342No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.15507883173946757No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC60.15507883173946757No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC60.15507883173946757No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC50.12923235978288963No Hit
AGCCTAGGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGA50.12923235978288963No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT50.12923235978288963No Hit
AGCCTAAACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC50.12923235978288963No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAA40.10338588782631171No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAT40.10338588782631171No Hit
AGCCTAGACATCCACTGTTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC40.10338588782631171No Hit
AGCCTAGACATCCACAGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC40.10338588782631171No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC40.10338588782631171No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGCC40.10338588782631171No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC40.10338588782631171No Hit
AGCCTAGACATCCACTGCTGAACGTTGTTATCTAGAATAAAAGTAGAGAC40.10338588782631171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)