FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi715.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi715.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4849
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT215444.421530212414936No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGAACT57611.878737884099815No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT52010.723860589812332No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT50210.352650030934212No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT2895.959991750876469No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGGCCGGAGATGTGGTAATGATC430.8867807795421737No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTTAAAAGCAGAGACT280.5774386471437409No Hit
AGCCTAGACATCCACTGCTGGAGATGTGGTAATGATCCTTCCGCTGTCTC220.45370179418436796No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTC170.350587750051557No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGAATT160.32996494122499487No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT150.30934213239843267No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT150.30934213239843267No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACC140.28871932357187047No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT140.28871932357187047No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT120.24747370591874612No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT110.22685089709218398No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT100.20622808826562178No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATCTAGTTAAAAGCAGAGACT80.16498247061249743No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAGAAGCAGAGACT70.14435966178593523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTGTCTAGTTAAAAGCAGAGACT70.14435966178593523No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAGAGCAAGGACTT70.14435966178593523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTGGTTAAAAGCAGAGACT70.14435966178593523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAGAGCAAGAATT70.14435966178593523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTAAAAGCAGAGACT60.12373685295937306No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAGAGCAGAGACT60.12373685295937306No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGCTAAAAGCAGAGACT60.12373685295937306No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATCTAGTTAAAAGCAGAGACT60.12373685295937306No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGAACT50.10311404413281089No Hit
AGCCTAGACATCCATTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT50.10311404413281089No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCCAGTTAAAAGCAGAGACT50.10311404413281089No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTCAAAGCAGAGACT50.10311404413281089No Hit
AGACTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT50.10311404413281089No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGCCT50.10311404413281089No Hit
AGCCTAGACATACACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT50.10311404413281089No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATCTAGTTAAAAGCAGAGACT50.10311404413281089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)