FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5302
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT355867.10675216899283No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT4799.034326669181441No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT1613.036589966050547No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT1332.508487363259148No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC731.3768389287061487No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT530.999622783855149No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC400.7544322897019993No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT250.4715201810637495No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT230.4337985665786495No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGGACT170.3206337231233497No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAAGGACT170.3206337231233497No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT160.3017729158807997No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT160.3017729158807997No Hit
AGCCTAGACATCCACTGCTGCCGTGGTAATGATCCTTCCGCTGTCTCTTA160.3017729158807997No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT150.2829121086382497No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACC150.2829121086382497No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTTAAAAGCAAGGACT150.2829121086382497No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAGAGCAAGGACT140.2640513013956997No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGGACT140.2640513013956997No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGTTAAAAGCAAGGACT140.2640513013956997No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTTAAAAGCAAGGACT130.24519049415314978No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAGAAGCAAGGACT130.24519049415314978No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGGCT130.24519049415314978No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT130.24519049415314978No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTTAAAAGCAAGGACT110.2074688796680498No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTGAAAGCAAGGACT110.2074688796680498No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTACAAGCAAGGACT90.16974726518294983No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTCAAAAGCAAGGACT90.16974726518294983No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTTAAAAGCAAGGACT90.16974726518294983No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT80.15088645794039984No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTCAAAGCAAGGACT80.15088645794039984No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT80.15088645794039984No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT60.1131648434552999No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTTAGTTAAAAGCAAGGACT60.1131648434552999No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAAGGACT60.1131648434552999No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAGAGCAGAGACT60.1131648434552999No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGTTAAAAGCAAGGACT60.1131648434552999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)