FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT90229.593175853018373No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT66421.784776902887142No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT62520.50524934383202No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT1093.5761154855643045No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT812.65748031496063No Hit
AGCCTAGACATCCACTGCTGATGTGGTAATGATCCTTCCGCTGTCTCTTA441.4435695538057742No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT260.8530183727034121No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT240.7874015748031495No Hit
AGCCTAGACATCCACTGCTGTCTCGGGGCTCGGAGATGTGGTAATGATCC190.6233595800524935No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTGTCTAGTTAAAAGCAGAGACT160.5249343832020997No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT140.45931758530183725No Hit
AGCCTAGACATCCACTGCTGCCGAGTGGTAATGATCCTTCCGCTGTCTCT130.42650918635170604No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAACGACTT120.39370078740157477No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC110.36089238845144356No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT70.22965879265091863No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGGCT70.22965879265091863No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAAGGACT70.22965879265091863No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT60.19685039370078738No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT60.19685039370078738No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGAATT60.19685039370078738No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTTAAAAGCAAGAATT50.16404199475065617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAGAAGCAGAGACT50.16404199475065617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGTTAAAAGCAAGGACT50.16404199475065617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTTAAAAGCAAGGACT50.16404199475065617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGAATT40.13123359580052493No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTTAAAAGCAAGAATT40.13123359580052493No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT40.13123359580052493No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGAATT40.13123359580052493No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT40.13123359580052493No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT40.13123359580052493No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGGACT40.13123359580052493No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAGAGCAAGAATT40.13123359580052493No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGTTAAAAGCAAGGACT40.13123359580052493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)