FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC202369.25710373159876No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT2247.66860664156111No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT1394.758644299897295No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT321.0955152345087298No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC170.5819924683327627No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC130.4450530640191715No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC120.41081821294077375No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC100.3423485107839781No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC100.3423485107839781No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC100.3423485107839781No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC90.3081136597055803No Hit
AGCCTAGACATCCACTGCTGGGTGGTAATGATCCTTCCGCTGTCTCTTAT90.3081136597055803No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC90.3081136597055803No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC80.27387880862718245No Hit
AGCCTAGACATCCACTGCTGAAAGTTACTATCTAGTTAAAAGCAGAGACT80.27387880862718245No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC80.27387880862718245No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC80.27387880862718245No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAA70.23964395754878468No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT70.23964395754878468No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC70.23964395754878468No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC70.23964395754878468No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC70.23964395754878468No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC60.20540910647038688No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.20540910647038688No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC60.20540910647038688No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC60.20540910647038688No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC60.20540910647038688No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.20540910647038688No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGCC60.20540910647038688No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC50.17117425539198905No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC50.17117425539198905No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC50.17117425539198905No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT50.17117425539198905No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC50.17117425539198905No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGTAGAGAC50.17117425539198905No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC40.13693940431359122No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC40.13693940431359122No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAGGCAAGGACTT40.13693940431359122No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAACGTAGAGAC40.13693940431359122No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC40.13693940431359122No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGAATAAAAGTAGAGAC30.10270455323519344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAATAGAGAC30.10270455323519344No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.10270455323519344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGTAGAATAAAAGTAGAGAA30.10270455323519344No Hit
AGCCCAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.10270455323519344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGTAGAGAC30.10270455323519344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGGTATCTAGAATAAAAGTAGAGAC30.10270455323519344No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.10270455323519344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCACGGACTT30.10270455323519344No Hit
GCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT30.10270455323519344No Hit
AGCCTAGACATCCACCGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.10270455323519344No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.10270455323519344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTTGAGAC30.10270455323519344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)