FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi686.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi686.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4131
Sequences flagged as poor quality0
Sequence length301
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC350084.72524812394093No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC260.6293875574921327No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC200.4841442749939482No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC180.4357298474945534No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC180.4357298474945534No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC160.38731541999515856No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC160.38731541999515856No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC150.36310820624546114No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC150.36310820624546114No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC140.3389009924957638No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC120.29048656499636893No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC120.29048656499636893No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC120.29048656499636893No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC110.2662793512466715No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC110.2662793512466715No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC100.2420721374969741No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC100.2420721374969741No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC90.2178649237472767No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC90.2178649237472767No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC90.2178649237472767No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC80.19365770999757928No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC80.19365770999757928No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC80.19365770999757928No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGCC80.19365770999757928No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC70.1694504962478819No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGTAGAGAC70.1694504962478819No Hit
AGCCTAGACATCCACCGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC70.1694504962478819No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC70.1694504962478819No Hit
AGCCTAGACATCCACTGCTGAACGTTGTTATCTAGAATAAAAGTAGAGAC70.1694504962478819No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGAATAAAAGTAGAGAC60.14524328249818447No Hit
AGCCCAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.14524328249818447No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.14524328249818447No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC60.14524328249818447No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.14524328249818447No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT50.12103606874848705No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT50.12103606874848705No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC50.12103606874848705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)