FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5152
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT168532.70574534161491No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT125624.37888198757764No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC125524.35947204968944No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTAAAAGCAGGGACTT340.6599378881987578No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT260.5046583850931677No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT230.4464285714285714No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC220.42701863354037267No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGAGACTT140.2717391304347826No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT120.2329192546583851No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAGAGACT110.21350931677018634No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCCAGTAAAAGCAGGGACTT110.21350931677018634No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTAAAAGCAGGGACTT100.19409937888198758No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATCTAGTAAAAGCAGGGACTT100.19409937888198758No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT100.19409937888198758No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC100.19409937888198758No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC90.17468944099378883No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACCT90.17468944099378883No Hit
AGCCTAGACATACACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT80.15527950310559005No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC80.15527950310559005No Hit
AGACTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT80.15527950310559005No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAGAAGCAGAGACT80.15527950310559005No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTTAAAAGCAGAGACT80.15527950310559005No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC80.15527950310559005No Hit
AGCCTAGACATCCACTGCTGATAGTTGCTATCTAGTAAAAGCAGGGACTT80.15527950310559005No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGCAGAGAC70.1358695652173913No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGAATT70.1358695652173913No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTC70.1358695652173913No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAGAGACT70.1358695652173913No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAGAGCAGGGACTT70.1358695652173913No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT70.1358695652173913No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGGCTT70.1358695652173913No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAGGCAGGGACTT60.11645962732919254No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATCTAGTAAAAGCAGGGACTT60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTAAAAGCAGGGACTT60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTGTCTAGTAAAAGCAGGGACTT60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTAAAAGCAGGGACTT60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACC60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTTAAAAGCAGAGACT60.11645962732919254No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAGAGACT60.11645962732919254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)