FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOChi668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOChi668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5907
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC442374.87726426273912No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT5178.752327746741155No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC340.5755882850854919No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC270.4570848146267141No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC250.4232266802099204No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC230.3893685457931268No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC220.37243947858473No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC220.37243947858473No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT200.33858134416793634No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC180.30472320975114275No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC180.30472320975114275No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC170.28779414254274593No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC170.28779414254274593No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC160.27086507533434906No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC150.25393600812595224No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC150.25393600812595224No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGCC150.25393600812595224No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAATTAGAGAC150.25393600812595224No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC140.23700694091755545No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC140.23700694091755545No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC130.2200778737091586No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.2200778737091586No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.20314880650076178No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC120.20314880650076178No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.20314880650076178No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC120.20314880650076178No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.20314880650076178No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGCAGAGAC110.186219739292365No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC100.16929067208396817No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC90.15236160487557138No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC90.15236160487557138No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGCAGAGAC80.13543253766717453No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC80.13543253766717453No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGAATAAAAGCAGAGAC70.11850347045877772No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT70.11850347045877772No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.10157440325038089No Hit
TGAGTAACCGCAACATAGTGAACGGCTATTCGACGCTGACTCATCCTGTC60.10157440325038089No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAAAC60.10157440325038089No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT60.10157440325038089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)