FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH911.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH911.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5448
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT497291.26284875183553No Hit
AGCCTAGAAATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT190.34875183553597655No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT150.27533039647577096No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAACAACTTT140.25697503671071953No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT130.23861967694566813No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT120.22026431718061676No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACGTT80.14684287812041116No Hit
AGCCTAGATATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT80.14684287812041116No Hit
AGCCTAGACATCCACTGATGAAAGTTTGTGGAAGTGAACGCAAAAACTTT80.14684287812041116No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT80.14684287812041116No Hit
AGTCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.12848751835535976No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGTAAAAACTTT70.12848751835535976No Hit
AGCCTAGACATTCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.12848751835535976No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGCAGTGAACGCAAAAACTTT70.12848751835535976No Hit
AGCCTAGACATCCACTGCTGCTCGGAGATGTGGTAATGATCCTTCCGCTG60.11013215859030838No Hit
AGCCTAGGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTT60.11013215859030838No Hit
AGCATAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT60.11013215859030838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)