FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5897
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT184431.270137357978633No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT164327.861624554858405No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC151525.691029336950994No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT1592.6962862472443616No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT691.1700864846532135No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT380.6443954553162625No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC240.4069866033576394No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT170.28828217737832795No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT120.2034933016788197No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.1526199762591148No Hit
AGCCTAGACATCCACTGCTGCCGACGACGTGGTAATGATCCTTCCGCTGT90.1526199762591148No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT80.13566220111921318No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT70.11870442597931151No Hit
AGCCTAGACATACACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT70.11870442597931151No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT60.10174665083940986No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC60.10174665083940986No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.10174665083940986No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT60.10174665083940986No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)