FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6528
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT557585.40134803921569No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC1802.7573529411764706No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT280.428921568627451No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT210.3216911764705882No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT210.3216911764705882No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT200.30637254901960786No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT180.2757352941176471No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT180.2757352941176471No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC160.24509803921568626No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT160.24509803921568626No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT160.24509803921568626No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT150.2297794117647059No Hit
AGCCTAGACATCCACTGCTGGAAGTTTGTGGAAGTGAACGCAAAAACTTT140.2144607843137255No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT130.19914215686274508No Hit
AGCCTAGAAATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT130.19914215686274508No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT120.1838235294117647No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT110.16850490196078433No Hit
AGCCTAGACATCCACTGCTGAAAGCTTGTGGAAGTGAACGCAAAAACTTT110.16850490196078433No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCGAAAACTTT110.16850490196078433No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTGAACGCAAAAACTTT110.16850490196078433No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAGAACTTT100.15318627450980393No Hit
AGCCTAGACATCCACTGCTGACAGTTTGTGGAAGTGAACGCAAAAACTTT100.15318627450980393No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT100.15318627450980393No Hit
CCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTCG90.13786764705882354No Hit
AGCCTAGACATCCACTGATGAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.13786764705882354No Hit
AGCCTAGACATCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC90.13786764705882354No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT90.13786764705882354No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT90.13786764705882354No Hit
AGCCTAGACATCCACTGCTGAGAGTTTGTGGAAGTGAACGCAAAAACTTT80.12254901960784313No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAACAACTTT80.12254901960784313No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAGCTTT80.12254901960784313No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT80.12254901960784313No Hit
AGCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC70.10723039215686275No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCACAAACTTT70.10723039215686275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)