FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH639.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH639.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3072
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT91929.915364583333332No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT71323.209635416666664No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT45414.778645833333334No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT3079.993489583333332No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC1856.022135416666666No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT300.9765625No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGAAAGGACTT250.8138020833333334No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT180.5859375No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT120.390625No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATTGATTAAAACCAAAGACTT110.35807291666666663No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT60.1953125No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAGGACTT60.1953125No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT60.1953125No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT60.1953125No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTC50.16276041666666669No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAAGGACT50.16276041666666669No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACC50.16276041666666669No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAGAACCAAAGACTT50.16276041666666669No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT40.13020833333333331No Hit
AGCCTAGACATCCACTGCTGAAATTTGTTATCTAGTTAAAAGCAAGGACT40.13020833333333331No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGACTAAAACCAAAGACTT40.13020833333333331No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAGAGACTT40.13020833333333331No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGGTTAAAACCAAAGACTT40.13020833333333331No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT40.13020833333333331No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT40.13020833333333331No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC40.13020833333333331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)