FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH637.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH637.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6693
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT489673.15105333930973No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT4256.3499178245928585No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC4116.140744060959212No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT741.105632750634992No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT330.49305244285073957No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT280.41834752726729424No Hit
AGCCTAGACATCCACTGCTGGAAGTTTGTGGAAGTGAACGCAAAAACTTT250.37352457791722693No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC240.3585835948005379No Hit
AGCCTAGACATCCACTGCTGGAGTGTGGTAATGATCCTTCCGCTGTCTCT240.3585835948005379No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT200.29881966233378154No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT200.29881966233378154No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT190.2838786792170925No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT180.2689376961004034No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT170.25399671298371435No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT170.25399671298371435No Hit
AGCCTAGAAATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT170.25399671298371435No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAACAACTTT160.23905572986702522No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAATGCAAAAACTTT160.23905572986702522No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT150.22411474675033619No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT140.20917376363364712No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCGAAAACTTT110.16435081428357987No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT100.14940983116689077No Hit
AGCCTAGACATCCACTGCTGAAAGCTTGTGGAAGTGAACGCAAAAACTTT100.14940983116689077No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT100.14940983116689077No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.1344688480502017No Hit
CGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.1344688480502017No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTCT90.1344688480502017No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTGAACGCAAAAACTTT90.1344688480502017No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT90.1344688480502017No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT80.11952786493351261No Hit
AGCCTAGACATCCACTGATGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.10458688181682356No Hit
AGCCTAGACATCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC70.10458688181682356No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.10458688181682356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)