FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT128839.10139647844566No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACTT108432.9083181542198No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT35610.807528840315724No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC300.9107468123861567No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTGGATTAAAACCAAGACTTT190.5768063145112325No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT170.5160898603521554No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT160.4857316332726169No Hit
AGCCTAGACATCCACTGCTGTAAGAGACAGTGGTAATGATCCTTCCGCTG150.45537340619307837No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT150.45537340619307837No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAATTAAAACCAAGAACT140.42501517911353975No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT90.273224043715847No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT90.273224043715847No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACCT80.24286581663630846No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACTT70.21250758955676988No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACTT70.21250758955676988No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGAGACTT70.21250758955676988No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGCCTT70.21250758955676988No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACTC70.21250758955676988No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTAAAAGCAAAGACTT70.21250758955676988No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTAAAAGCAAGGACTT60.18214936247723132No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT60.18214936247723132No Hit
AGCCTAGACATCCACTGCTGGATGTGGTAATGATCCTTCCGCTGTCTCTT60.18214936247723132No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAAAGCAAAGACTT50.15179113539769276No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAGGCAAGGACTT50.15179113539769276No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTAAAAGCAAGGACTT50.15179113539769276No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACCT50.15179113539769276No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGGCTT50.15179113539769276No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTAAAAGCAAGGACTT50.15179113539769276No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAAAGCAAGGACTT40.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTC40.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAACCAAGAGAC40.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTAAAAGCAAAGACTT40.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGGCTT40.12143290831815423No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT40.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCGAAGACTT40.12143290831815423No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT40.12143290831815423No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)