FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4920
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC397280.73170731707317No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT1222.4796747967479673No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC200.40650406504065045No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC190.3861788617886179No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC190.3861788617886179No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC190.3861788617886179No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC180.3658536585365854No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC170.34552845528455284No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC160.3252032520325203No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC160.3252032520325203No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC160.3252032520325203No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC150.3048780487804878No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC150.3048780487804878No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC150.3048780487804878No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC150.3048780487804878No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC140.2845528455284553No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGGATTAAAACCAAAAACTT130.26422764227642276No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT120.24390243902439024No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC120.24390243902439024No Hit
AGCCTAGACATCCACTGCTGAAAGTTATTATTGATTAAACAGACGACTTT120.24390243902439024No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC120.24390243902439024No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT120.24390243902439024No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC110.22357723577235775No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC110.22357723577235775No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC100.20325203252032523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC100.20325203252032523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC100.20325203252032523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGCC100.20325203252032523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC90.1829268292682927No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC90.1829268292682927No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATCAAAGTAGAGAC90.1829268292682927No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC80.16260162601626016No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGAATAAAAGTAGAGAC80.16260162601626016No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.14227642276422764No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGCGAC70.14227642276422764No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC70.14227642276422764No Hit
AGCCTAGACATCCACCGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC70.14227642276422764No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC70.14227642276422764No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC70.14227642276422764No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGACTAAAAGTAGAGAC60.12195121951219512No Hit
AGCCTAGACATCCACTGCCGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.12195121951219512No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGTGAC60.12195121951219512No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.12195121951219512No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.12195121951219512No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.12195121951219512No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGAATAAAAGTAGAGAC50.10162601626016261No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC50.10162601626016261No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC50.10162601626016261No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGTAGAGAC50.10162601626016261No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT50.10162601626016261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)