FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8830
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT511457.916194790486976No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC266030.124575311438278No Hit
CGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT240.2718006795016987No Hit
AGCCTAGACATCAACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT220.2491506228765572No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT220.2491506228765572No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT200.22650056625141565No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT180.20385050962627407No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT150.16987542468856173No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT140.15855039637599094No Hit
AGCATAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT130.14722536806342015No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.14722536806342015No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAACAACTTT120.13590033975084936No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT120.13590033975084936No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.13590033975084936No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC120.13590033975084936No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT120.13590033975084936No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC110.1245753114382786No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC110.1245753114382786No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT110.1245753114382786No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT110.1245753114382786No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT110.1245753114382786No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT100.11325028312570783No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAGAACTTT90.10192525481313704No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.10192525481313704No Hit
AGCCTAGACATCCACTGATGAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.10192525481313704No Hit
AGCCTAGAAATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.10192525481313704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)