FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH603.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH603.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5289
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT269851.011533371147664No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT128824.352429570807335No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC3486.579693703913783No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT531.002079788239743No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT520.9831726224238986No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATCTAGTTAAAAGCAGAGACT200.37814331631688414No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGCTAAAAGCAGAGACT180.3403289846851957No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT150.28360748723766305No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT150.28360748723766305No Hit
AGCCTAGACATCCACTGCTGAAAGTTATTATCTAGTTAAAAGCAAGGACT140.2647003214218189No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTAAAAGCAGAGACT140.2647003214218189No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAGAAGCAGAGACT110.20797882397428627No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT110.20797882397428627No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACC110.20797882397428627No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT100.18907165815844207No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAGAGCAGAGACT100.18907165815844207No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT100.18907165815844207No Hit
AGCCTAGACATCCACTGCTGACGAGTGGTAATGATCCTTCCGCTGTCTCT100.18907165815844207No Hit
AGCCTAGACATCAACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT100.18907165815844207No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT90.17016449234259784No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT90.17016449234259784No Hit
AGCATAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT80.15125732652675364No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTTAAAAGCAGAGACT80.15125732652675364No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT80.15125732652675364No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTGAAAGCAGAGACT80.15125732652675364No Hit
AGCCTAGACATACACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT80.15125732652675364No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATCTAGTTAAAAGCAGAGACT80.15125732652675364No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTTAAAAGCAGAGACT70.13235016071090944No Hit
AGCCTAGACATCCACTGCTGTAAGTTGCTATCTAGTTAAAAGCAGAGACT70.13235016071090944No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT70.13235016071090944No Hit
AGCCTAGACATCCACTGCTGCCGACGACGTGGTAATGATCCTTCCGCTGT70.13235016071090944No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT60.11344299489506524No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT60.11344299489506524No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTTAAAAGCAAGGACT60.11344299489506524No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTGGTTAAAAGCAGAGACT60.11344299489506524No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATCTAGTTAAAAGCAGAGACT60.11344299489506524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)