FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH598.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH598.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5782
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC451478.06987201660324No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT3375.828433068142512No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT1242.1445866482186093No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC250.4323763403666551No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC230.3977862331373227No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC200.3459010722933241No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC200.3459010722933241No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC190.3286060186786579No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC190.3286060186786579No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC180.3113109650639917No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC180.3113109650639917No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC170.2940159114493255No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC160.2767208578346593No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC160.2767208578346593No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC150.2594258042199931No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC150.2594258042199931No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC150.2594258042199931No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC140.24213075060532688No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC130.2248356969906607No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.20754064337599448No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC110.19024558976132827No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC110.19024558976132827No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC110.19024558976132827No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGCC100.17295053614666206No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGAATAAAAGCAGAGAC100.17295053614666206No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC100.17295053614666206No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGCAGAGAC90.15565548253199585No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATCAAAGCAGAGAC90.15565548253199585No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC90.15565548253199585No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAT80.13836042891732964No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC80.13836042891732964No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATACAAGCAGAGAC70.12106537530266344No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.12106537530266344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC60.10377032168799724No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGCAGAGAC60.10377032168799724No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAAAC60.10377032168799724No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT60.10377032168799724No Hit
AGCCTAGACATCCACCGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.10377032168799724No Hit
AGCCTAGACATCCACTGCCGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.10377032168799724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)